Which of the following correctly defines a cofactor?

A cofactor is defined as a nonprotein molecule that is necessary for the proper functioning of an enzyme. Cofactors can be inorganic, such as metal ions (like zinc, iron, or magnesium), or they can be organic molecules, often referred to as coenzymes (like vitamins). They assist in various biochemical reactions by stabilizing the enzyme structure or participating directly in the reactions being catalyzed.

This definition is crucial in understanding enzyme activity, as many enzymes require these additional molecules to achieve their functional state. Without the appropriate cofactor, some enzymes may be inactive. Understanding this helps in grasping how enzymes interact with substrates and how various factors influence metabolic pathways in biological systems.